Gene CCR5 e Resistência ao HIV- Resumo da Mutação CYS101TER

CURSO DE BIOMEDICINA BACHARELADO DISCIPLINA Citogenética Molecular e Biologia Molecular DOCENTE Tarcísio André Amorim de Carvalho DISCENTE TURMABM06MA DATA 2020 GENE CCR5 Site GENE no NCBI httpswwwncbinlmnihgovgene1234 Site OMIM no NCBI httpsomimorgentry601373 Variação genética no site OMIM allelic variants Nome da mutação CCR5 CYS101TER Código da mutação rs1800560 httpswwwensemblorgHomosapiensVariationSummaryvrs1800560toggleHGVSna mesopen RESUMO Este gene codifica um membro da família de receptores beta quimiocinas que se prevê ser uma proteína sete transmembrana semelhante aos receptores acoplados à proteína G Esta proteína é expressa por células T e macrófagos e é conhecida por ser um importante coreceptor para vírus com tropismo para macrófagos incluindo HIV para entrar nas células hospedeiras Alelos defeituosos desse gene têm sido associados à resistência à infecção pelo HIV Os ligantes deste receptor incluem proteína quimioatraente de monócitos 2 MCP2 proteína 1 alfa inflamatória de macrófagos MIP1 alfa proteína 1 beta inflamatória de macrófagos MIP1 beta e regulada na ativação da proteína T normal expressa e secretada RANTES A expressão desse gene também foi detectada em uma linha celular promieloblástica sugerindo que essa proteína pode desempenhar um papel na proliferação e diferenciação de linhagens de granulócitos Este gene está localizado na região do agrupamento de genes do receptor de quimiocinas Um polimorfismo alélico neste gene resulta em alelos funcionais e não funcionais o genoma de referência representa o alelo funcional Duas variantes de transcrição que codificam a mesma proteína foram encontradas para este gene FENÓTIPO GENÓTIPO A variação genética é uma mutação que troca um aminoácido CISTEINA por um códon de finalização impedindo o funcionamento correto do receptor e criando uma possível resistência à infecção pelo HIV AMINOÁCIDO CYS PROTEÍNA NORMAL SUSCEPTÍVEL À INFECÇÃO PELO HIV TERMINAÇÃO PROTEÍNA DEFEITUOSA RESISTÊNCIA À INFECÇÃO PELO HIV SEQUÊNCIA DO GENE CCR5 httpswwwncbinlmnihgovnuccoreNG0126371from5001to11065reportgenban k PRIMERS SELECIONADOS POR MEIO DO PROGRAMA PRIMER 3 PLUS httpsprimer3pluscomcgibindevprimer3pluscgi Sequências em amarelo éxons mRNA Sequencia grifada região codificante CDs Sequência em negrito primers Sequência em vermelho variação genética mutação CTTCAGATAGATTATATCTGGAGTGAAGAATCCTGCCACCTATGTATCTGGCATAGTGTGAGTCCTCATAAATGCTTACTG GTTTGAAGGGCAACAAAATAGTGAACAGAGTGAAAATCCCCACTAAGATCCTGGGTCCAGAAAAAGATGGGAAACCTGTTT AGCTCACCCGTGAGCCCATAGTTAAAACTCTTTAGACAACAGGTTGTTTCCGTTTACAGAGAACAATAATATTGGGTGGTG AGCATCTGTGTGGGGGTTGGGGTGGGATAGGGGATACGGGGAGAGTGGAGAAAAAGGGGACACAGGGTTAATGTGAAGTCC AGGATCCCCCTCTACATTTAAAGTTGGTTTAAGTTGGCTTTAATTAATAGCAACTCTTAAGATAATCAGAATTTTCTTAAC CTTTTAGCCTTACTGTTGAAAAGCCCTGTGATCTTGTACAAATCATTTGCTTCTTGGATAGTAATTTCTTTTACTAAAATG TGGGCTTTTGACTAGATGAATGTAAATGTTCTTCTAGCTCTGATATCCTTTATTCTTTATATTTTCTAACAGATTCTGTGT AGTGGGATGAGCAGAGAACAAAAACAAAATAATCCAGTGAGAAAAGCCCGTAAATAAACCTTCAGACCAGAGATCTATTCT CTAGCTTATTTTAAGCTCAACTTAAAAAGAAGAACTGTTCTCTGATTCTTTTCGCCTTCAATACACTTAATGATTTAACTC CACCCTCCTTCAAAAGAAACAGCATTTCCTACTTTTATACTGTCTATATGATTGATTTGCACAGCTCATCTGGCCAGAAGA GCTGAGACATCCGTTCCCCTACAAGAAACTCTCCCCGGTAAGTAACCTCTCAGCTGCTTGGCCTGTTAGTTAGCTTCTGAG ATGAGTAAAAGACTTTACAGGAAACCCATAGAAGACATTTGGCAAACACCAAGTGCTCATACAATTATCTTAAAATATAAT CTTTAAGATAAGGAAAGGGTCACAGTTTGGAATGAGTTTCAGACGGTTATAACATCAAAGATACAAAACATGATTGTGAGT GAAAGACTTTAAAGGGAGCAATAGTATTTTAATAACTAACAATCCTTACCTCTCAAAAGAAAGATTTGCAGAGAGATGAGT CTTAGCTGAAATCTTGAAATCTTATCTTCTGCTAAGGAGAACTAAACCCTCTCCAGTGAGATGCCTTCTGAATATGTGCCC ACAAGAAGTTGTGTCTAAGTCTGGTTCTCTTTTTTCTTTTTCCTCCAGACAAGAGGGAAGCCTAAAAATGGTCAAAATTAA TATTAAATTACAAACGCCAAATAAAATTTTCCTCTAATATATCAGTTTCATGGCACAGTTAGTATATAATTCTTTATGGTT CAAAATTAAAAATGAGCTTTTCTAGGGGCTTCTCTCAGCTGCCTAGTCTAAGGTGCAGGGAGTTTGAGACTCACAGGGTTT AATAAGAGAAAATTCTCAGCTAGAGCAGCTGAACTTAAATAGACTAGGCAAGACAGCTGGTTATAAGACTAAACTACCCAG AATGCATGACATTCATCTGTGGTGGCAGACGAAACATTTTTTATTATATTATTTCTTGGGTATGTATGACAACTCTTAATT GTGGCAACTCAGAAACTACAAACACAAACTTCACAGAAAATGTGAGGATTTTACAATTGGCTGTTGTCATCTATGACCTTC CCTGGGACTTGGGCACCCGGCCATTTCACTCTGACTACATCATGTCACCAAACATCTGATGGTCTTGCCTTTTAATTCTCT TTTCGAGGACTGAGAGGGAGGGTAGCATGGTAGTTAAGAGTGCAGGCTTCCCGCATTCAAAATCGGTTGCTTACTAGCTGT GTGGCTTTGAGCAAGTTACTCACCCTCTCTGTGCTTCAAGGTCCTTGTCTGCAAAATGTGAAAAATATTTCCTGCCTCATA AGGTTGCCCTAAGGATTAAATGAATGAATGGGTATGATGCTTAGAACAGTGATTGGCATCCAGTATGTGCCCTCGAGGCCT CTTAATTATTACTGGCTTGCTCATAGTGCATGTTCTTTGTGGGCTAACTCTAGCGTCAATAAAAATGTTAAGACTGAGTTG CAGCCGGGCATGGTGGCTCATGCCTGTAATCCCAGCATTCTAGGAGGCTGAGGCAGGAGGATCGCTTGAGCCCAGGAGTTC GAGACCAGCCTGGGCAACATAGTGTGATCTTGTATCTATAAAAATAAACAAAATTAGCTTGGTGTGGTGGCGCCTGTAGTC CCCAGCCACTTGGAGGGGTGAGGTGAGAGGATTGCTTGAGCCCGGGATGGTCCAGGCTGCAGTGAGCCATGATCGTGCCAC TGCACTCCAGCCTGGGCGACAGAGTGAGACCCTGTCTCACAACAACAACAACAACAACAAAAAGGCTGAGCTGCACCATGC TTGACCCAGTTTCTTAAAATTGTTGTCAAAGCTTCATTCACTCCATGGTGCTATAGAGCACAAGATTTTATTTGGTGAGAT GGTGCTTTCATGAATTCCCCCAACAGAGCCAAGCTCTCCATCTAGTGGACAGGGAAGCTAGCAGCAAACCTTCCCTTCACT ACAAAACTTCATTGCTTGGCCAAAAAGAGAGTTAATTCAATGTAGACATCTATGTAGGCAATTAAAAACCTATTGATGTAT AAAACAGTTTGCATTCATGGAGGGCAACTAAATACATTCTAGGACTTTATAAAAGATCACTTTTTATTTATGCACAGGGTG GAACAAGATGGATTATCAAGTGTCAAGTCCAATCTATGACATCAATTATTATACATCGGAGCCCTGCCAAAAAATCAATGT GAAGCAAATCGCAGCCCGCCTCCTGCCTCCGCTCTACTCACTGGTGTTCATCTTTGGTTTTGTGGGCAACATGCTGGTCAT CCTCATCCTGATAAACTGCAAAAGGCTGAAGAGCATGACTGACATCTACCTGCTCAACCTGGCCATCTCTGACCTGTTTTT CCTTCTTACTGTCCCCTTCTGGGCTCACTATGCTGCCGCCCAGTGGGACTTTGGAAATACAATGTGTCAACTCTTGACA GGGCTCTATTTTATAGGCTTCTTCTCTGGAATCTTCTTCATCATCCTCCTGACAATCGATAGGTACCTGGCTGTCGTCCAT GCTGTGTTTGCTTTAAAAGCCAGGACGGTCACCTTTGGGGTGGTGACAAGTGTGATCACTTGGGTGGTGGCTGTGTTTGCG TCTCTCCCAGGAATCATCTTTACCAGATCTCAAAAAGAAGGTCTTCATTACACCTGCAGCTCTCATTTTCCATACAGTCAG TATCAATTCTGGAAGAATTTCCAGACATAAAGATAGTCATCTTGGGGCTGGTCCTGCCGCTGCTTGTCATGGTCATCTGCT Comentário TAAC1 MUTAÇÃO TROCA DE TIMINA POR ADENINA TROCA DO AMINOÁCIDO CISTEINA POR UM CÓDON DE FINALIZAÇÃO ACTCGGGAATCCTAAAAACTCTGCTTCGGTGTCGAAATGAGAAGAAGAGGCACAGGGCTGTGAGGCTTATCTTCACCATCA TGATTGTTTATTTTCTCTTCTGGGCTCCCTACAACATTGTCCTTCTCCTGAACACCTTCCAGGAATTCTTTGGCCTGAATA ATTGCAGTAGCTCTAACAGGTTGGACCAAGCTATGCAGGTGACAGAGACTCTTGGGATGACGCACTGCTGCATCAACCCCA TCATCTATGCCTTTGTCGGGGAGAAGTTCAGAAACTACCTCTTAGTCTTCTTCCAAAAGCACATTGCCAAACGCTTCTGCA AATGCTGTTCTATTTTCCAGCAAGAGGCTCCCGAGCGAGCAAGCTCAGTTTACACCCGATCCACTGGGGAGCAGGAAATAT CTGTGGGCTTGTGACACGGACTCAAGTGGGCTGGTGACCCAGTCAGAGTTGTGCACATGGCTTAGTTTTCATACACAGCCT GGGCTGGGGGTGGGGTGGGAGAGGTCTTTTTTAAAAGGAAGTTACTGTTATAGAGGGTCTAAGATTCATCCATTTATTTGG CATCTGTTTAAAGTAGATTAGATCTTTTAAGCCCATCAATTATAGAAAGCCAAATCAAAATATGTTGATGAAAAATAGCAA CCTTTTTATCTCCCCTTCACATGCATCAAGTTATTGACAAACTCTCCCTTCACTCCGAAAGTTCCTTATGTATATTTAAAA GAAAGCCTCAGAGAATTGCTGATTCTTGAGTTTAGTGATCTGAACAGAAATACCAAAATTATTTCAGAAATGTACAACTTT TTACCTAGTACAAGGCAACATATAGGTTGTAAATGTGTTTAAAACAGGTCTTTGTCTTGCTATGGGGAGAAAAGACATGAA TATGATTAGTAAAGAAATGACACTTTTCATGTGTGATTTCCCCTCCAAGGTATGGTTAATAAGTTTCACTGACTTAGAACC AGGCGAGAGACTTGTGGCCTGGGAGAGCTGGGGAAGCTTCTTAAATGAGAAGGAATTTGAGTTGGATCATCTATTGCTGGC AAAGACAGAAGCCTCACTGCAAGCACTGCATGGGCAAGCTTGGCTGTAGAAGGAGACAGAGCTGGTTGGGAAGACATGGGG AGGAAGGACAAGGCTAGATCATGAAGAACCTTGACGGCATTGCTCCGTCTAAGTCATGAGCTGAGCAGGGAGATCCTGGTT GGTGTTGCAGAAGGTTTACTCTGTGGCCAAAGGAGGGTCAGGAAGGATGAGCATTTAGGGCAAGGAGACCACCAACAGCCC TCAGGTCAGGGTGAGGATGGCCTCTGCTAAGCTCAAGGCGTGAGGATGGGAAGGAGGGAGGTATTCGTAAGGATGGGAAGG AGGGAGGTATTCGTGCAGCATATGAGGATGCAGAGTCAGCAGAACTGGGGTGGATTTGGGTTGGAAGTGAGGGTCAGAGAG GAGTCAGAGAGAATCCCTAGTCTTCAAGCAGATTGGAGAAACCCTTGAAAAGACATCAAGCACAGAAGGAGGAGGAGGAGG TTTAGGTCAAGAAGAAGATGGATTGGTGTAAAAGGATGGGTCTGGTTTGCAGAGCTTGAACACAGTCTCACCCAGACTCCA GGCTGTCTTTCACTGAATGCTTCTGACTTCATAGATTTCCTTCCCATCCCAGCTGAAATACTGAGGGGTCTCCAGGAGGAG ACTAGATTTATGAATACACGAGGTATGAGGTCTAGGAACATACTTCAGCTCACACATGAGATCTAGGTGAGGATTGATTAC CTAGTAGTCATTTCATGGGTTGTTGGGAGGATTCTATGAGGCAACCACAGGCAGCATTTAGCACATACTACACATTCAATA AGCATCAAACTCTTAGTTACTCATTCAGGGATAGCACTGAGCAAAGCATTGAGCAAAGGGGTCCCATAGAGGTGAGGGAAG CCTGAAAAACTAAGATGCTGCCTGCCCAGTGCACACAAGTGTAGGTATCATTTTCTGCATTTAACCGTCAATAGGCAAAGG GGGGAAGGGACATATTCATTTGGAAATAAGCTGCCTTGAGCCTTAAAACCCACAAAAGTACAATTTACCAGCCTCCGTATT TCAGACTGAATGGGGGTGGGGGGGGCGCCTTAGGTACTTATTCCAGATGCCTTCTCCAGACAAACCAGAAGCAACAGAAAA AATCGTCTCTCCCTCCCTTTGAAATGAATATACCCCTTAGTGTTTGGGTATATTCATTTCAAAGGGAGAGAGAGAGGTTTT TTTCTGTTCTGTCTCATATGATTGTGCACATACTTGAGACTGTTTTGAATTTGGGGGATGGCTAAAACCATCATAGTACAG GTAAGGTGAGGGAATAGTAAGTGGTGAGAACTACTCAGGGAATGAAGGTGTCAGAATAATAAGAGGTGCTACTGACTTTCT CAGCCTCTGAATATGAACGGTGAGCATTGTGGCTGTCAGCAGGAAGCAACGAAGGGAAATGTCTTTCCTTTTGCTCTTAAG TTGTGGAGAGTGCAACAGTAGCATAGGACCCTACCCTCTGGGCCAAGTCAAAGACATTCTGACATCTTAGTATTTGCATAT TCTTATGTATGTGAAAGTTACAAATTGCTTGAAAGAAAATATGCATCTAATAAAAAACACCTTCTAAAATAA GENE APOE Site GENE no NCBI httpswwwncbinlmnihgovgene348 Site OMIM no NCBI httpsomimorgentry107741searchAPOEhighlightapoe Variação genética no site OMIM allelic variants Nome da mutação APOE2 variant Código da mutação rs429358 httpswwwensemblorgHomosapiensVariationExplore dbcoreph60505r194490818444909184vrs429358vdbvariationvf201931519 Resumo A proteína codificada por este gene é a principal apoproteína do quilomícron Ligase a um receptor específico do fígado e da célula periférica e é essencial para o catabolismo normal dos constituintes das lipoproteínas ricas em triglicerídeos Este gene mapeia para o cromossomo 19 em um cluster com os genes relacionados da apolipoproteína C1 e C2 Mutações nesse gene resultam em disbetalipoproteinemia familiar ou hiperlipoproteinemia tipo III HLP III na qual o aumento do colesterol e dos triglicerídeos plasmáticos é consequência do comprometimento da depuração de quilomícrons e remanescentes de VLDL Sequência do gene httpswwwncbinlmnihgovnuccore163954918 Primers selecionados httpswwwprimer3pluscomindexhtmlUUID88c9830301ef47a183f1 0529f1aeeea0 1 TGGAGAGCTG GTCTACCACC GGCGGCCTGG AGAGGAGGGC ACTGTCATGT 51 CTCTAGCTGG GAAATACACA TGTGAGCCTG GCGCCTGGGT CCGAGGGTGG 101 AGGGGCTGGG CCCCTGGACT CCTCCTGGGT CTGAGGGAGG ACGGGCTAGG 151 GCCCTGGACA CTCAGGTCTG AGGGAGGAGG CCTGGGTTCC CAGATGCCCA 201 AATCCCCTTG GTAATGAGAC CCCGCCTCCA CCCCACTCTC TGACAGTGAA 251 CAACTGGTTG GCAACGGTAA CGTTGGGCCA GGCGGGCATG CACGCAACAT 301 ACTACCACAA AGCCAGTGAC CAGGTGAGTG GGTGCAGGGA CTAGCTGGTG 351 CTGCCAGGGG CTGCTGGGCC TGGAAGTCCA GGTGGGGCCA CTTGCTAATT 401 CTCATGTGTT GCTCCGGCCC CTCCAGCTGC AGGTGGGTGT GGAGTTTGAG 451 GCCAGCACAA GGATGCAGGA CACCAGCGTC TCCTTCGGGT ACCAGCTGGA 501 CCTGCCCAAG GCCAACCTCC TCTTCAAAGG TAAAGGTCTC GGTTCCCCTA 551 CGCGGGAAAC AGGCAGGAGG TGACTCAACT CTGAGTGGAT GTGTGGGCCA 601 CCACAGGTGC TGGAGGACAG TGTGCTGCCA CCCTGTGGGC CTCCACATTA 651 CCAGGGAACA CTTGTTAAAA GGTAGGTGGG GCCGGGTGCG GTGGCTCACG 701 CCTGTAATCC CAGCACTTTG GGAGGCCAAG GCGGGCCGAG GTAAGGAGAT 751 TGAGACCATC CTGGCTAACA CGGTGAAACT CCGTCTCTAC TAAAAATACA 801 AAAACAAAAT TAGCCGGGTG TGGTTGCGGG TGCCTATAGT CCCAACTACT 851 GAGGCTGAGG CGGGAAAATG GTATGAACCC AGGAGGCGGA GCTTGCGGTG 901 AGCCGAGATC GTGCCACCGC ACTCCAGCCT GGGTGACAGA GCAAGACTCC 951 ATCTCAAAAA AAAAAAAGTA GGTGGACAAC CCTCTACTAT GTTTTATGCT 1001 TGGAAAAAAA AAGTAGGTAG AGCAGCCAGG CGTGGTGACT CACGCCTGTA 1051 ATCCCAGCAT TTTGGGAGGC CAAGCCAGGT AGAATACTTG AGGCCAGGAG 1101 TTGGAGACCA GCCTGGCCAA CGTGGTGAAA TCCCCTCTCT ACTAAAAGTA 1151 CAAAAATTAG CCAGGTGTGG TAGCGTGCTG CAACTGTAGT CCCCGCTACT 1201 TAGGAGGCTG AGGCACAAGA ATCACTTGAA CCTGGGAGGC GGAGGTTGCA 1251 GGGAGTTGAG ACTGCACCAC TGCACTCCAG CCTGGGTGAC AGAGTGAGAC 1301 TCCATCTCCA AAAAAAATAA AATGAAATAA ATAAATAAAT GTTAAAAAAA 1351 ATCTGGTGGA GCATCTGATG GGTGTTTGGG CCAAGCTGGA GCTTTGTCCA 1401 TCCCCTCTTA TTTTTCTGCA CTTGACTCTC TTATTTTTCT GAGACTGGTC 1451 TCCCTCTGTC GCCCAGGCTA GAGTGCAGCA GTGCAACTGC GGCTCACTGC 1501 AGCCTCCACC TCCCGGGCTC AAGCAGCCTT CCCACCTCAG CCTCCTGAGT 1551 AGCTAGGACC ACAGGTGTAT GCCACCAGGC CCAGCTAATT TTTTTGATAG 1601 TTTTGGGAGA CATGGGGGTT TCACCATGTT GCCCAGGCTG GTCTCGAACT 1651 CCTGGACTCA AGCCTTGGCC TCCCAAAGTG CTGGGATTAT AGGTGTGAGC 1701 CACCACACCC AGCCAGGGTA GAAGGCACTT TGGAAGCCTC GAGCCTGCCC 1751 CATTCATCTT ACGTTAGTGG AAACTGAGGC TTCCAGAGGT TTCAAGGTCA 1801 CAACTAAATC CAGAACCTCA TCTCAGGCAC ACTGGTCGTA GTCCCAATGT 1851 CCAGTCTTAA GTCTTCTTGG ATATCTGTGG CTCACAGATT TTGGGTGTTT 1901 GAGCCTCCTG CTGAGCACTG CTGGGGCCAC AGCGGTGACC AGCCCTGTCT 1951 TCACGGGACT CAGTGAGAGG AACAGATTCA TCCGCAGAGT GGGCAGGACT 2001 AGGTTGGGGG AACCCAGGGG TCTAGAGGGC TTTTCAGAGG GCAGGGGTCA 2051 CTGAGCGGAG AGCAGAGGAG GAGTGAGCCA TTTGCTCCAG CGTGAAGTTG 2101 TTGGTGTGAT GGGGTTTCAG GGTGGCAGGA GCAGTGTGGT TAAAGGTCTG 2151 GAAGCTGTCG GCATGTGGCT GGTATCCAAG GTGGCCAGGA ACTCTGCATG 2201 GATATGGTGG GAAGCTGGCA CGCCTCTCAC CTCAGCTCTT CCCTGCAGGC 2251 TCTGTGGATA GCAACTGGAT CGTGGGTGCC ACGCTGGAGA AGAAGCTCCC 2301 ACCCCTGCCC CTGACACTGG CCCTTGGGGC CTTCCTGAAT CACCGCAAGA 2351 ACAAGTTTCA GTGTGGCTTT GGCCTCACCA TCGGCTGAGC CCTCCTGGCC 2401 CCCGCCTTCC ACGCCCTTCC GATTCCACCT CCACCTCCAC CTCCCCCTGC 2451 CACAGAGGGG AGACCTGAGC CCCCCTCCCT TCCCTCCCCC CTTGGGGGTC 2501 GGGGGGGACA TTGGAAAGGA GGGACCCCGC CACCCCAGCA GCTGAGGAGG 2551 GGATTCTGGA ACTGAATGGC GCTTCGGGAT TCTGAGTAGC AGGGGCAGCA 2601 TGCCCAGTGG GCCTGGGGTC CCGGGAGGGA TTCCGGAATT GAGGGGCACG 2651 CAGGATTCTG AGCACCAGGG GCAGAGGCGG CCAGACAACC TCAGGGAGGA 2701 GTGTCCTGGC GTCCCCATCC TCCAAAGGGC CTGGGCCCGC CCCGAGGGGG 2751 CAGCGAGAGG AGCTTCCCCA TCCCCGGTCA GTCCACCCTG CCCCGTCCAC 2801 TTTCCCATCT CCTCGGTATA AATCATGTTT ATAAGTTATG GAAGAACCGG 2851 GACATTTTAC AGAAAAAAAA CAAAAAACAA CAAAAAATAT ACGTGGGAAA 2901 AAAAACGATG GGAGGCCTCC GTTTTCTCAA GTGTGTCTGG CCTGTTTTGA 2951 GCATTTCATC CGGAGTCTGG CCGCCCTGAC CTTCCCCCAG CCGCCTGCAG 3001 GGGGCGCCAG AGGGCCGGAG CACGGAAAGC AGCGGATCCT TGATGCTGCC 3051 TTAAGTCCGG CTCAGAGGGG CGCAGCGTGG CCTGGGGTCG CTATCTTCCC 3101 ATCCGGAACA TCTGCCCTGC TGGGGGACAC TACGGGCCTT CCCTTGCCTG 3151 AGGGTAGGGT CTCAAGGTCA CTTGCCCCCA GCTTGACCTG GCCGGAGTGG 3201 CTATAGAGGA CTTTGTCCCT GCAGACTGCA GCAGCAGAGA TGACACTGTC 3251 TCTGAGTGCA GAGATGGGGG CAGGGAGCTG GGAGAGGGTT CAAGCTACTG 3301 GAACAGCTTC AGAACAACTA GGGTACTAGG AACTGCTGTG TCAGGGAGAA 3351 GGGGCTCAAG GACTCGCAGG CCTGGGAGGA GGGGCCTAGG CCAGCCATGG 3401 GAGTTGGGTC ACCTGTGTCT GAGGACTTGG TGCTGTCTGG ATTTTGCCAA 3451 CCTAGGGCTG GGGTCAGCTG ATGCCCACCA CGACTCCCGA GCCTCCAGGA 3501 ACTGAAACCC TGTCTGCCCC CAGGGTCTGG GGAAGGAGGC TGCTGAGTAG 3551 AACCAACCCC AGGTTACCAA CCCCACCTCA GCCACCCCTT GCCAGCCAAA 3601 GCAAACAGGC CCGGCCCGGC ACTGGGGGTT CCTTCTCGAA CCAGGAGTTC 3651 AGCCTCCCCT GACCCGCAGA ATCTTCTGAT CCCACCCGCT CCAGGAGCCA 3701 GGAATGAGTC CCAGTCTCTC CCAGTTCTCA CTGTGTGGTT TTGCCATTCG 3751 TCTTGCTGCT GAACCACGGG TTTCTCCTCT GAAACATCTG GGATTTATAA 3801 CAGGGCTTAG GAAAGTGACA GCGTCTGAGC GTTCACTGTG GCCTGTCCAT 3851 TGCTAGCCCT AACATAGGAC CGCTGTGTGC CAGGGCTGTC CTCCATGCTC 3901 AATACACGTT AGCTTGTCAC CAAACATACC CGTGCCGCTG CTTTCCCAGT 3951 CTGATGAGCA AAGGAACTTG ATGCTCAGAG AGGACAAGTC ATTTGCCCAA 4001 GGTCACACAG CTGGCAACTG GCAGAGCCAG GATTCACGCC CTGGCAATTT 4051 GACTCCAGAA TCCTAACCTT AACCCAGAAG CACGGCTTCA AGCCCCTGGA 4101 AACCACAATA CCTGTGGCAG CCAGGGGGAG GTGCTGGAAT CTCATTTCAC 4151 ATGTGGGGAG GGGGCTCCCC TGTGCTCAAG GTCACAACCA AAGAGGAAGC 4201 TGTGATTAAA ACCCAGGTCC CATTTGCAAA GCCTCGACTT TTAGCAGGTG 4251 CATCATACTG TTCCCACCCC TCCCATCCCA CTTCTGTCCA GCCGCCTAGC 4301 CCCACTTTCT TTTTTTTCTT TTTTTGAGAC AGTCTCCCTC TTGCTGAGGC 4351 TGGAGTGCAG TGGCGAGATC TCGGCTCACT GTAACCTCCG CCTCCCGGGT 4401 TCAAGCGATT CTCCTGCCTC AGCCTCCCAA GTAGCTAGGA TTACAGGCGC 4451 CCGCCACCAC GCCTGGCTAA CTTTTGTATT TTTAGTAGAG ATGGGGTTTC 4501 ACCATGTTGG CCAGGCTGGT CTCAAACTCC TGACCTTAAG TGATTCGCCC 4551 ACTGTGGCCT CCCAAAGTGC TGGGATTACA GGCGTGAGCT ACCGCCCCCA 4601 GCCCCTCCCA TCCCACTTCT GTCCAGCCCC CTAGCCCTAC TTTCTTTCTG 4651 GGATCCAGGA GTCCAGATCC CCAGCCCCCT CTCCAGATTA CATTCATCCA 4701 GGCACAGGAA AGGACAGGGT CAGGAAAGGA GGACTCTGGG CGGCAGCCTC 4751 CACATTCCCC TTCCACGCTT GGCCCCCAGA ATGGAGGAGG GTGTCTGTAT 4801 TACTGGGCGA GGTGTCCTCC CTTCCTGGGG ACTGTGGGGG GTGGTCAAAA 4851 GACCTCTATG CCCCACCTCC TTCCTCCCTC TGCCCTGCTG TGCCTGGGGC 4901 AGGGGGAGAA CAGCCCACCT CGTGACTGGG GGCTGGCCCA GCCCGCCCTA 4951 TCCCTGGGGG AGGGGGCGGG ACAGGGGGAG CCCTATAATT GGACAAGTCT 5001 GGGATCCTTG AGTCCTACTC AGCCCCAGCG GAGGTGAAGG ACGTCCTTCC 5051 CCAGGAGCCG GTGAGAAGCG CAGTCGGGGG CACGGGGATG AGCTCAGGGG 5101 CCTCTAGAAA GAGCTGGGAC CCTGGGAACC CCTGGCCTCC AGGTAGTCTC 5151 AGGAGAGCTA CTCGGGGTCG GGCTTGGGGA GAGGAGGAGC GGGGGTGAGG 5201 CAAGCAGCAG GGGACTGGAC CTGGGAAGGG CTGGGCAGCA GAGACGACCC 5251 GACCCGCTAG AAGGTGGGGT GGGGAGAGCA GCTGGACTGG GATGTAAGCC 5301 ATAGCAGGAC TCCACGAGTT GTCACTATCA TTTATCGAGC ACCTACTGGG 5351 TGTCCCCAGT GTCCTCAGAT CTCCATAACT GGGGAGCCAG GGGCAGCGAC 5401 ACGGTAGCTA GCCGTCGATT GGAGAACTTT AAAATGAGGA CTGAATTAGC 5451 TCATAAATGG AACACGGCGC TTAACTGTGA GGTTGGAGCT TAGAATGTGA 5501 AGGGAGAATG AGGAATGCGA GACTGGGACT GAGATGGAAC CGGCGGTGGG 5551 GAGGGGGTGG GGGGATGGAA TTTGAACCCC GGGAGAGGAA GATGGAATTT 5601 TCTATGGAGG CCGACCTGGG GATGGGGAGA TAAGAGAAGA CCAGGAGGGA 5651 GTTAAATAGG GAATGGGTTG GGGGCGGCTT GGTAAATGTG CTGGGATTAG 5701 GCTGTTGCAG ATAATGCAAC AAGGCTTGGA AGGCTAACCT GGGGTGAGGC 5751 CGGGTTGGGG CCGGGCTGGG GGTGGGAGGA GTCCTCACTG GCGGTTGATT 5801 GACAGTTTCT CCTTCCCCAG ACTGGCCAAT CACAGGCAGG AAGATGAAGG 5851 TTCTGTGGGC TGCGTTGCTG GTCACATTCC TGGCAGGTAT GGGGGCGGGG 5901 CTTGCTCGGT TCCCCCCGCT CCTCCCCCTC TCATCCTCAC CTCAACCTCC 5951 TGGCCCCATT CAGGCAGACC CTGGGCCCCC TCTTCTGAGG CTTCTGTGCT 6001 GCTTCCTGGC TCTGAACAGC GATTTGACGC TCTCTGGGCC TCGGTTTCCC 6051 CCATCCTTGA GATAGGAGTT AGAAGTTGTT TTGTTGTTGT TGTTTGTTGT 6101 TGTTGTTTTG TTTTTTTGAG ATGAAGTCTC GCTCTGTCGC CCAGGCTGGA 6151 GTGCAGTGGC GGGATCTCGG CTCACTGCAA GCTCCGCCTC CCAGGTCCAC 6201 GCCATTCTCC TGCCTCAGCC TCCCAAGTAG CTGGGACTAC AGGCACATGC 6251 CACCACACCC GACTAACTTT TTTGTATTTT CAGTAGAGAC GGGGTTTCAC 6301 CATGTTGGCC AGGCTGGTCT GGAACTCCTG ACCTCAGGTG ATCTGCCCGT 6351 TTCGATCTCC CAAAGTGCTG GGATTACAGG CGTGAGCCAC CGCACCTGGC 6401 TGGGAGTTAG AGGTTTCTAA TGCATTGCAG GCAGATAGTG AATACCAGAC 6451 ACGGGGCAGC TGTGATCTTT ATTCTCCATC ACCCCCACAC AGCCCTGCCT 6501 GGGGCACACA AGGACACTCA ATACATGCTT TTCCGCTGGG CGCGGTGGCT 6551 CACCCCTGTA ATCCCAGCAC TTTGGGAGGC CAAGGTGGGA GGATCACTTG 6601 AGCCCAGGAG TTCAACACCA GCCTGGGCAA CATAGTGAGA CCCTGTCTCT 6651 ACTAAAAATA CAAAAATTAG CCAGGCATGG TGCCACACAC CTGTGCTCTC 6701 AGCTACTCAG GAGGCTGAGG CAGGAGGATC GCTTGAGCCC AGAAGGTCAA 6751 GGTTGCAGTG AACCATGTTC AGGCCGCTGC ACTCCAGCCT GGGTGACAGA 6801 GCAAGACCCT GTTTATAAAT ACATAATGCT TTCCAAGTGA TTAAACCGAC 6851 TCCCCCCTCA CCCTGCCCAC CATGGCTCCA AAGAAGCATT TGTGGAGCAC 6901 CTTCTGTGTG CCCCTAGGTA CTAGATGCCT GGACGGGGTC AGAAGGACCC 6951 TGACCCACCT TGAACTTGTT CCACACAGGA TGCCAGGCCA AGGTGGAGCA 7001 AGCGGTGGAG ACAGAGCCGG AGCCCGAGCT GCGCCAGCAG ACCGAGTGGC 7051 AGAGCGGCCA GCGCTGGGAA CTGGCACTGG GTCGCTTTTG GGATTACCTG 7101 CGCTGGGTGC AGACACTGTC TGAGCAGGTG CAGGAGGAGC TGCTCAGCTC 7151 CCAGGTCACC CAGGAACTGA GGTGAGTGTC CCCATCCTGG CCCTTGACCC 7201 TCCTGGTGGG CGGCTATACC TCCCCAGGTC CAGGTTTCAT TCTGCCCCTG 7251 TCGCTAAGTC TTGGGGGGCC TGGGTCTCTG CTGGTTCTAG CTTCCTCTTC 7301 CCATTTCTGA CTCCTGGCTT TAGCTCTCTG GAATTCTCTC TCTCAGCTTT 7351 GTCTCTCTCT CTTCCCTTCT GACTCAGTCT CTCACACTCG TCCTGGCTCT 7401 GTCTCTGTCC TTCCCTAGCT CTTTTATATA GAGACAGAGA GATGGGGTCT 7451 CACTGTGTTG CCCAGGCTGG TCTTGAACTT CTGGGCTCAA GCGATCCTCC 7501 CGCCTCGGCC TCCCAAAGTG CTGGGATTAG AGGCATGAGC CACCTTGCCC 7551 GGCCTCCTAG CTCCTTCTTC GTCTCTGCCT CTGCCCTCTG CATCTGCTCT 7601 CTGCATCTGT CTCTGTCTCC TTCTCTCGGC CTCTGCCCCG TTCCTTCTCT 7651 CCCTCTTGGG TCTCTCTGGC TCATCCCCAT CTCGCCCGCC CCATCCCAGC 7701 CCTTCTCCCC GCCTCCCACT GTGCGACACC CTCCCGCCCT CTCGGCCGCA 7751 GGGCGCTGAT GGACGAGACC ATGAAGGAGT TGAAGGCCTA CAAATCGGAA 7801 CTGGAGGAAC AACTGACCCC GGTGGCGGAG GAGACGCGGG CACGGCTGTC 7851 CAAGGAGCTG CAGGCGGCGC AGGCCCGGCT GGGCGCGGAC ATGGAGGACG 7901 TGTGCGGCCG CCTGGTGCAG TACCGCGGCG AGGTGCAGGC CATGCTCGGC 7951 CAGAGCACCG AGGAGCTGCG GGTGCGCCTC GCCTCCCACC TGCGCAAGCT 8001 GCGTAAGCGG CTCCTCCGCG ATGCCGATGA CCTGCAGAAG CGCCTGGCAG 8051 TGTACCAGGC CGGGGCCCGC GAGGGCGCCG AGCGCGGCCT CAGCGCCATC 8101 CGCGAGCGCC TGGGGCCCCT GGTGGAACAG GGCCGCGTGC GGGCCGCCAC 8151 TGTGGGCTCC CTGGCCGGCC AGCCGCTACA GGAGCGGGCC CAGGCCTGGG 8201 GCGAGCGGCT GCGCGCGCGG ATGGAGGAGA TGGGCAGCCG GACCCGCGAC 8251 CGCCTGGACG AGGTGAAGGA GCAGGTGGCG GAGGTGCGCG CCAAGCTGGA 8301 GGAGCAGGCC CAGCAGATAC GCCTGCAGGC CGAGGCCTTC CAGGCCCGCC 8351 TCAAGAGCTG GTTCGAGCCC CTGGTGGAAG ACATGCAGCG CCAGTGGGCC 8401 GGGCTGGTGG AGAAGGTGCA GGCTGCCGTG GGCACCAGCG CCGCCCCTGT 8451 GCCCAGCGAC AATCACTGAA CGCCGAAGCC TGCAGCCATG CGACCCCACG 8501 CCACCCCGTG CCTCCTGCCT CCGCGCAGCC TGCAGCGGGA GACCCTGTCC 8551 CCGCCCCAGC CGTCCTCCTG GGGTGGACCC TAGTTTAATA AAGATTCACC 8601 AAGTTTCACG CATCTGCTGG CCTCCCCCTG TGATTTCCTC TAAGCCCCAG 8651 CCTCAGTTTC TCTTTCTGCC CACATACTGG CCACACAATT CTCAGCCCCC 8701 TCCTCTCCAT CTGTGTCTGT GTGTATCTTT CTCTCTGCCC TTTTTTTTTT 8751 TTTTAGACGG AGTCTGGCTC TGTCACCCAG GCTAGAGTGC AGTGGCACGA 8801 TCTTGGCTCA CTGCAACCTC TGCCTCTTGG GTTCAAGCGA TTCTGCTGCC 8851 TCAGTAGCTG GGATTACAGG CTCACACCAC CACACCCGGC TAATTTTTGT 8901 ATTTTTAGTA GAGACGAGCT TTCACCATGT TGGCCAGGCA GGTCTCAAAC 8951 TCCTGACCAA GTGATCCACC CGCCGGCCTC CCAAAGTGCT GAGATTACAG 9001 GCCTGAGCCA CCATGCCCGG CCTCTGCCCC TCTTTCTTTT TTAGGGGGCA 9051 GGGAAAGGTC TCACCCTGTC ACCCGCCATC ACAGCTCACT GCAGCCTCCA