Bioquímica Enzimas

TABELA 6-5 Algumas reações que servem de catalisadores enzimáticos Enzima Catalisada pela dissociação de RNA Nucleosídeos 5′-difenifósfatos 5′-AMP AMP AMP componente desaminado Dissulfeto de polipeptídeos (glutationas) LISINA LISYL-AMP RESÍDUO DE DOAÇÃO DE GENERALIDADE ATA DE FORMAÇÃO DE Enzima Ácido Glutâmico (Glu) Aspartato de lisina Arginina (Asp) Cisteína Serina Tirosina COO− CH2− COO− CH2− NH3+ Cisteína HS NH CH2− Serina OH NH3+ Cisteína HS−O− TABELA 6-9 Efeitos de inibidores reversíveis na Vmax aparente e Km aparente Tipo de Inibidor Vmax aparente Km aparente Sem inibidor Vmax Km Competitivo Vmax αKm Incompetitivo Vmax/α′ Km/α′ Misto Vmax/α′ αKm/α′ Lys147 abstracts one hydrogen atom from the γ-carbon atom, resulting in an anionic enolate intermediate. This enolate nucleophilically attacks (R) 2-hydroxyglutarate (also known as D -2-hydroxyglutarate. (G) CH APOS F-Phenylpyruvat (a) Lys147 abstracts one hydrogen atom from the γ-carbon atom, resulting in an anionic enolate intermediate. This enolate nucleophilically attacks (b) Lys147 facilitates the formation of enolate from γ-ketoisocaproate, and PLP stabilizes the enolate through charge delocalization. E Fosfhidroxipropanoate MECHANISM FIGURE 5. Reaction de short steps catalisado pellest Hydroxylase de argininaA uvorocoluna, UG . Asparagineakarta keto-isocaproate (Dz-ketopropanoate: D-2-(Lazgo.PLP)-enoato, IV1BEnzymealelaenameloctron B-ketopropanoate: 24/j-ray-E multiseuted: PDESTPOS) " One proton generated by NNMT remains on the planar nitrogen. This protects against nucleophilic attack. An anionic enolate is favored and stabilized by PLP. Lys147 can protonate the enolate intermediate to generate the ketone. Methionine S-methyl-5'-deoxyadenosine One proton generated by NNMT remains on the planar nitrogen. This protects against nucleophilic attack. An anionic enolate is favored and stabilized by PLP. Threomine ketone Lys147 can protonate the enolate intermediate to generate the ketone. CYS-C bounding specifical harmony FIGURE 6 MECHANISM FIGURE 6 Reaction de EventsBio Ist Residues are clutch by dots and well NC(NF. Methylthio.Amadenosine to pen peptide by the lessen of CH41.isodiumners. Losis de projection le pidirectili Fac le rana enzeyme hogy cio 2Aatured logis de project plug enzeyme table F4 layers